Test of Antibacterial Effectiveness of Cat's Whisker Leaf Extract (Orthosiphon stamineus) against Streptococcus bacteria mutans
Rizky Mulyanti, Member Reni Purba, Dian Soraya Tanjung, Shania Salsabila
Abstract
Introduction; Streptococcus mutans is a pathogenic bacteria and the main cause of dental caries. Swe needs a preventive measure for dental caries with natural antibacterial ingredients with cat whiskers. Aim; determine the effect of antibacterial effectiveness of cat whiskers leaf extract against streptococcus mutans bacteria. Method; The study used a laboratory experimental design with posttest only control group in vitro. The data was collected by measuring the diameter of the zone of inhibition using a shear caliper. Then, the data were analyzed using one-way ANOVA and posthoc LSD statistical tests. Result; that the average diameter of the inhibition zone of cat whiskers leaf extract (Orthosiphon stamineus) antibacterial with a concentration of 50%, 25%, 12.5%, 6.25%, 3.125% against Streptococcus mutans. bacteria were 11.50 ± 0.38 mm, 10.78 ± 0.22 mm, 9.90 ± 0.13 mm, 9.10 ± 0.08 mm and 8.25 ± 0.25 mm, while the control was negative. In this study, DMSO does not have an inhibitory diameter (0 mm). Conclusion; that there is antibacterial effectiveness of cat whiskers leaf extract against Streptococcus mutans bacteria
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References
Adnyana, I. K., Setiawan, F., & Insanu, M. (2013). From ethnopharmacology to clinical study of Orthosiphon stamineus Benth. International Journal of Pharmacy and Pharmaceutical Sciences, 5(3), 66–73.
Al-Shahrani. (2019). Microbiology of dental caries. A Literature Review. Ann Med Health Sci Res, 9.
Alhabdan, Y. A., Albeshr, A. G., Yenugadhati, N., & Jradi, H. (2018). Prevalence of dental caries and associated factors among primary school children: a population-based cross-sectional study in Riyadh, Saudi Arabia. Environmental Health and Preventive Medicine, 23(1), 1–14.
Alshawsh, M. A., Abdulla, M. A., Ismail, S., Amin, Z. A., Qader, S. W., Hadi, H. A., & Harmal, N. S. (2012). Free radical scavenging, antimicrobial and immunomodulatory activities of Orthosiphon stamineus. Molecules, 17(5), 5385–5395.
Ashraf, K., Halim, H., Lim, S. M., Ramasamy, K., & Sultan, S. (2020). In vitro antioxidant, antimicrobial and antiproliferative studies of four different extracts of Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea. Saudi Journal of Biological Sciences, 27(1), 417–432.
Ashraf, K., Sultan, S., & Adam, A. (2018). Orthosiphon stamineus Benth. is an outstanding food medicine: Review of phytochemical and pharmacological activities. Journal of Pharmacy & Bioallied Sciences, 10(3), 109.
Azizan, N., Mohd Said, S., Zainal Abidin, Z., & Jantan, I. (2017). Composition and antibacterial activity of the essential oils of Orthosiphon stamineus Benth and Ficus deltoidea Jack against pathogenic oral bacteria. Molecules, 22(12), 2135.
Haque, M., Sartelli, M., & Haque, S. Z. (2019). Dental infection and resistance—global health consequences. Dentistry Journal, 7(1), 22.
Hossain, M. A., & Rahman, S. M. M. (2015). Isolation and characterisation of flavonoids from the leaves of medicinal plant Orthosiphon stamineus. Arabian Journal of Chemistry, 8(2), 218–221.
Kartini, K., Dewi, E. R., Achmad, F., Jayani, N. I. E., Hadiyat, M. A., & Avanti, C. (2020). Thin Layer Chromatography Fingerprinting and Clustering of Orthosiphon stamineus Benth. from Different Origins. Pharmacognosy Journal, 12(1).
Khusuma, A., Safitri, Y., Yuniarni, A., & Rizki, K. (2019). Uji Teknik Difusi Menggunakan Kertas Saring Media Tampung Antibiotik dengan Escherichia Coli Sebagai Bakteri Uji. Jurnal Kesehatan Prima, 13(2), 151–155.
Kolay, S. K., & Kumar, S. (2019). Prevalence of dental caries: Children in Darbhanga population. Group, 4068, 27–35.
Krzyściak, W., Kościelniak, D., Papież, M., Jurczak, A., & Vyhouskaya, P. (2017). Methods of biotyping of Streptococcus mutans species with the routine test as a prognostic value in early childhood caries. Evidence-Based Complementary and Alternative Medicine, 2017.
Lemos, J. A., Palmer, S. R., Zeng, L., Wen, Z. T., Kajfasz, J. K., Freires, I. A., Abranches, J., & Brady, L. J. (2019). The biology of Streptococcus mutans. Gram‐Positive Pathogens, 435–448.
Nor, N. S. M., & Yasin, R. A. M. (2018). Antibacterial activity of Orthosiphon stamineus towards Staphylococcus aureus and methicilin resistant Stahpylococcus aureus (MRSA). J. Chem. Pharma. Sci., 11.
Rostinawati, T., Aryani, H., & Iskandar, Y. (2018). Identification of bacteria causing dental caries through genetic testing and activity assay of toothpastes. Journal of Pharmaceutical Sciences and Research, 10(3), 511–513.
Rukmana, R. M., & Mulyowati, T. (2015). Aktivitas Antibakteri dari Ekstrak Etanolik Daun Kumis Kucing (Orthosiphon stamineus) pada Bakteri Streptococcus pyogenes dan Salmonella thypi. Biomedika, 8(2), 15–18.
Singh, M. K., Gidwani, B., Gupta, A., Dhongade, H., Kaur, C. D., Kashyap, P. P., & Tripathi, D. K. (2015). A review of the medicinal plants of genus Orthosiphon (Lamiaceae). Int J Biol Chem, 9(6), 318–331.
stamineus Benth, O. (2019). Indonesian Journal of Science and Education. Indonesian Journal of Science and Education, 3(1), 26–33.
Takahashi, N., & Nyvad, B. (2016). Ecological hypothesis of dentin and root caries. Caries Research, 50(4), 422–431.
Tandra, T. A., Khairunissa, S., Sim, M., & Florenly, F. (2020). Efek Penambahan Nanokitosan 1% Kedalam Berbagai Konsentrasi Ekstrak Kulit Kelengkeng Streptococcus Mutans. Jurnal Ilmiah Kesehatan Sandi Husada, 11(1), 403–412.
WHO. (2020). Oral health conditions. http://www.who.in/new-rooms/fact-sheets/detail/oral-health
Young, D. A., Nový, B. B., Zeller, G. G., Hale, R., Hart, T. C., Truelove, E. L., Ekstrand, K. R., Featherstone, J. D. B., Fontana, M., & Ismail, A. (2015). The American Dental Association caries classification system for clinical practice: a report of the American Dental Association Council on Scientific Affairs. The Journal of the American Dental Association, 146(2), 79–86.
Yulianti, R., Nugraha, D. A., & Nurdianti, L. (2015). Formulasi Sediaan Sabun Mandi Cair Ekstrak Daun Kumis Kucing (Orthosiphon Aristatus (Bl) Miq.). Kartika: Jurnal Ilmiah Farmasi, 3(2), 1–11.
Zainal-Abidin, Z., Abdul-Wahab, N. A., Ghazi-Ahmad, M. K., & Mohd-Said, S. (2017). In vitro antibacterial activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis. Journal of Agricultural Science, 9(13), 112–121.
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