Bone Marrow-Derived Mesenchymal Stem Cell (BM-MSC) Sebagai Sumber Alternatif Sel Blastema Terhadap Regenerasi Anggota Tubuh

Kuntum Sureda

  • Kuntum Sureda Universitas Lampung
Keywords: Blastema cell, Bone marrow-derived mesenchymal stem cell, Limb regeneration


Limb regeneration is initiated by blastema cells (BC) and related genes that are only present in mammals confined to the distal fingertips of the phalangeal terminals and neonates. If amputated to proximal phalangeal, regeneration will fail due to lack of blastema cells (BC) in adults and difficulty in isolating and using blastema cells (BC) from neonates. BM-MSC has succeeded in increasing its ability to regenerate various wound tissue and its healing properties, because of this BM-MSC is used as an alternative cell source that produces blastema cells (BC). The original blastema cells (BC) that isolated from neontus and blastema cells (BC) in BM-MSC were compared to the ability of colony formation, cell proliferation, alkaline phosphatase activity (ALP), calcium supply, and osteogenic gene expression. The ability to form colonies was significantly higher in BM-MSC compared to original BC (P <0.05). Alizarin red stain (ARS), calcium and ALP tests show higher levels of mineral deposition in BM-MSCs. The qRT-PCR analysis revealed cells from both sources were ready to differentiate into mesodermal lineages. The rate of expression of osteblastic markers shows the capasity of bone differences is higher at BM-MSC at all time points.


Download data is not yet available.

Author Biography

Kuntum Sureda, Universitas Lampung

Pendidikan Dokter Fakultas Kedokteran


Bandyopadhyay A, Tsuji K, Cox K, Harfe BD, Rosen V, Tabin CJ. 2006. Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genet. 2(12): e216.

Boyette LB, Creasey OA, Guzik L, Lozito T, Tuan RS. 2014. Human bone marrow-derived mesenchymal stem cells display enhanced clonogenicity but impaired differentiation with hypoxic preconditioning. Stem Cells Transl Med.3(2): 241-254

Bryant SV, Endo T, Gardiner DM. 2002. Vertebrate limb regeneration and the origin of limb stem cells. Int J Dev Biol. 46(7): 887-896.

Dinsmore CE. 1996. Urodele limb and tail regeneration in early biological thought: an essay on scientific controversy and social change. Int J Dev Biol 40:621-7; PMID:8877433

Duque G. 2008. Bone and fat connection in the aging bone. Curr Opin Rheumatol. 20(4): 429-434.

Fernando WA, Leininger E, Simkin J, Li N, Malcolm CA, Sathyamoorthi S, et al. 2011. Wound healing and blastema formation in regenerating digit tips of adult mice. Dev Biol. 350(2): 301-310.

Franceschi RT, Xiao G. 2003. Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways. J Cell Biochem. 88(3): 446-454.

Han M, Yang X, Farrington JE, Muneoka K. 2003. Digit regeneration is regulated by Msx1 and BMP4 in fetal mice. Development. 130(21): 5123-5132.

Han MJ, An JY, Kim WS. 2001. Expression patterns of Fgf-8 during development and limb regeneration of the axolotl. Dev Dyn. 220(1): 40-48.

Ide H. 2012. Bone pattern formation in mouse limbs after amputation at the forearm level. Dev Dyn. 241(3): 435-441.

Jeon O, Rhie JW, Kwon IK, Kim JH, Kim BS, Lee SH. 2008. In vivo bone formation following transplantation of human adipose-derived stromal cells that are not differentiated osteogenically. Tissue Eng Part A. 14(8): 1285-1294

Masaki H, Ide H. 2007. Regeneration potency of mouse limbs. Dev Growth Differ. 49(2): 89-98.

Muraoka K, Sassoon D. 1992. Molecular aspects of regeneration in developing vertebrate limbs. Dev Biol. 152(1): 37-49.

Odelberg SJ. 2004. Unraveling the molecular basis for regenerative cellular plasticity. PLoS Biol.; 2(8): E232.

Penfornis P, Pochampally R. 2011. Isolation and expansion of mesenchymal stem cells/multipotential stromal cells from human bone marrow. Methods Mol Biol.; 698: 11-21.

Shih-Chang N, Zhu H, Ivanka de Soysa T, Shinoda G, Seligson MT, Tsanov KM, et al. 2013. Lin28 enhances tissue repair by reprogramming cellular metabolism. Cell. 155(4): 778-792.

Spallanzani L. Nouvelles Recherches. 2009. Part 1-2: SurLes Decouvertes Microscopiques Et La Generation Des Corps Organises (1769), pg 680. Whitefish, Montana: Kessinger Publishing.

Sugawara Y, Suzuki K, Koshikawa M, Ando M, Iida J. 2002. The necessity of enzymatic activity of alkaline phosphatase for mineralization of osteoblastic cells. Jpn J Pharmacol. 88(3): 262-269.

Tamura K, Ohno S, Yokoyama H. 2010. Limb blastema cell: a stem cell for morphological regeneration. Dev Growth Differ. 52(1): 89-99.

Tanaka EM. 2003. Cell differentiation and cell fate during urodele tail and limb regeneration. Curr Opin Genet Dev.; 13(5): 497-501

How to Cite
Sureda, K. (2019). Bone Marrow-Derived Mesenchymal Stem Cell (BM-MSC) Sebagai Sumber Alternatif Sel Blastema Terhadap Regenerasi Anggota Tubuh. Jurnal Ilmiah Kesehatan Sandi Husada, 10(2), 325-330. Retrieved from

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.